Journal: BMC oral health

Article Title: Preparation and characterization of bovine dental pulp-derived extracellular matrix hydrogel for regenerative endodontic applications: an in vitro study.

doi: 10.1186/s12903-024-05004-z

Figure Lengend Snippet: Fig. 6 Growth factor content; (a) Comparison between P-ECM & P-ECM + HA hydrogels regarding TGF-β1 release showing increased release in P-ECM + HA at 0,14 and 28 days ; (b) bFGF release showing higher release in P-ECM + HA group in 1,5 and 14 days ; (c) BMP2 release showing nearly indetectable concentration in P -ECM + HA while detectable in P-ECM; (d) VEGF release showing increased release of P-ECM at 7 days timepoint (e, f, g&h) bar graph showing comparison between P-ECM pre-gel and zero time point hydrogel after gelation

Article Snippet: The levels of TGF-β1, bFGF, BMP-2, and VEGF growth factors were assayed in supernatants of P-ECM and P-ECM + HA hydrogels and in pre-gel of P-ECM, using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions [human bone morphogenetic protein 2 (BMP-2) immunoassay, R&D Systems Inc, USA]; [Transforming growth factor beta 1 (TGF-β1 ) immunoassay, Cloud Clones Corp, USA]; [ bovine basic fibroblast growth factor (bFGF) and bovine vascular endothelial growth factor (VEGF), BTlab, China].

Techniques: Comparison, Concentration Assay

Journal: PLoS ONE

Article Title: Three-Dimensional Neuroepithelial Culture from Human Embryonic Stem Cells and Its Use for Quantitative Conversion to Retinal Pigment Epithelium

doi: 10.1371/journal.pone.0054552

Figure Lengend Snippet: (A, B) Sections of transplanted retina in the central region adjacent to the injection site shown in DIC image (A) and immunostained to identify human nuclei (red) and DAPI (blue) (B). Donor RPE cells (red) were localized in the sub-retinal space integrated into the host RPE monolayer and generated monolayer-like structures (A, B, arrows). The ONL overlying the donor RPE monolayer was well preserved and contained 5–6 rows of nuclei (B, arrow heads). (C) Electron microscopic analyses of transplanted RPE cells. The dotted line outlined one RPE cell. Typical polarized RPE morphology with foldings at the basal membrane, a nucleus located at the basal side (labeled by N) and apically located melanin-containing melanosomes (some labeled by M) were observed. (D, E) Representative fluorescence images of central or peripheral region in the hESC-derived RPE cell transplanted retina. Dashed box showed the ONL. Following transplantation of donor RPE cells, central regions of the retina showed an increased ONL thickness with an average of 5–6 rows of nuclei (D) in contrast to the periphery with 1–2 rows of nuclei (E). (F) Quantitative data for the effect of different transplanted cells on ONL thickness. (G) Quantitative data for the protected area of ONL in the human RPE and fibroblast cell transplanted retina. In panels (F) and (G), 6 RCS rats transplanted with human ESC-derived RPE cells, 5 with human fibroblasts, 5 sham-injected, and 6 untreated controls were quantified. Data were presented as means±SD. GCL: ganglion cell layer, INL: inner nuclear layer, ONL: outer nuclear layer. n.s.: not significant, *: P<0.05, **: P<0.01, ***: P<0.001. Nuclei were counterstained with DAPI. Scale bars, 10 µm (A, B), 5 µm (C), 20 µm (D, E).

Article Snippet: Control eyes were transplanted with human fibroblasts (Lonza) (n = 5), sham-injected (PBS) (n = 8), or untreated (n = 7).

Techniques: Injection, Generated, Membrane, Labeling, Fluorescence, Derivative Assay, Transplantation Assay

Journal: Military Medical Research

Article Title: Regenerative medicine in China: main progress in different fields

doi: 10.1186/s40779-016-0096-z

Figure Lengend Snippet: Growth factors for external using products approved for marketing by the SFDA

Article Snippet: Beijing SL Pharmaceutical , 2002 , Lyophilized Recombinant Human Basic Fibroblast Growth Factor (rh-bFGF) , Chronic Cutaneous Ulcer and Burn Wounds , SFDA Approval No.S20020025.

Techniques: Recombinant, Eye Drops, Transplantation Assay, Injection